Facultade de Fisioterapia

A critical assessment of ultrasound-assisted extraction as sample pre-treatment for fast determination of multielements in seafood using Inductively Coupled Plasma Mass Spectrometry

Authors: Adrián García-Figueroa, Jorge Millos, Carlos Bendicho, Isela Lavilla

Journal: Microchemical Journal, 130: 458-464, 01/2017 | Impact Factor: 2.893

DOI: http://dx.doi.org/10.1016/j.microc.2016.11.002


In this work, the suitability of extracts obtained from seafood samples after ultrasound-assisted extraction for determining Cd, Cu, Mn, Ni, Pb and Zn by Inductively Coupled Plasma-Mass Spectrometry was evaluated. For this purpose, different strategies were studied: (i) dilution of extracts; (ii) clean-up of extracts with C18 cartridges; (iii) use of a flow injection system for introducing extracts in the instrument. Potential interferences from acid extractant, major elements and organic matter were assessed. Internal standard, measurement mode and signal drift were also studied. Validation was carried out with the certified reference material TORT-2 (lobster hepatopancreas). Recoveries were between 92 and 106%. Precision, expressed as relative standard deviation, ranged from 2 to 6%. By using diluted extracts, limits of detection were similar as compared to those obtained after microwave-assisted digestion, or even better if a clean-up of extracts is applied. Following this approach, seafood samples of common consumption (mussel and shrimp) were rapidly analyzed.