Facultade de Fisioterapia

Principles and developments of solid-phase microextraction (book chapter)

Authors: A. Kloskowski, Ł. Marcinkowski, Francisco Pena-Pereira; J. Namiesnik

Book: Analytical Microextraction Techniques, Ed. Bentham, pp. 167-218, 2016

ISBN: 978-1-68108-380-3

DOI: https://doi.org/10.2174/9781681083797117010008


Sample preparation has been commonly considered a critical step of the analytical process. In this sense, remarkable efforts have been made to develop efficient sample preparation techniques which could overcome the limitations of conventional approaches. Since its inception in the early 1990’s, solid-phase microextraction (SPME) has become a widespread miniaturized sample preparation technique for extraction and preconcentration of target analytes from a large variety of matrices. Interestingly, sampling, extraction, enrichment and sample introduction can be integrated into a single step in SPME. This book chapter focuses on the basic principles and current state of the art of SPME. Specifically, both thermodynamic and kinetic aspects of the SPME technique are discussed in detail. In addition, those experimental variables that show a paramount role in the extraction process, and should therefore be optimized and controlled for optimal performance, are considered. Valuable contributions that enabled the development of this solventless technique and current challenges are identified. Other related SPME devices, such as internally cooled SPME, in-tube SPME and membrane SPME, are also described.